Label the MacConkey agar plates. E. faecalis is usually a nonpathogenic intestinal microorganism. The test indicated that P. aureus, S. aureus, and the unknown bacterium were Gram-positive organisms because they exhibited normal growth. June 26, 2021. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. Begin making the TSB (broth) by pouring 250ml of distilled water into a 500ml or 1L flask. Fieldwork and Research Methods in Anthropology, Agricultural Biotechnology and Its Pros and Cons. Biochemical Tests for Staphylococcus Organisms June 26, 2021. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. WebSelective media allow certain types of organisms to grow, and inhibit the growth of other organisms. WebSelective Media. result of the dyes metachromatic properties and also due to the robust acid end product test of In addition, it differentiates those organisms that grow based on whether they can carry out particular chemical reactions. State the usefulness of Columbia CNA agar and MacConkey agar. Wallace, J. S., & Jones, K. (1996). Test #1: Lab Experiment. growth to support their growth. NOT all Gram negative bacteria produce the same AMOUNT of acidic compounds if they can ferment lactose or sucrose, Poor to NO growth = StudyCorgi. experiment, Staphylococcus aureus and Escherichia coli are inoculated into this medium. section gives a vivid description and a deeper explanation on these responses and reasons behind It I spherical shaped(coca) and a Possibly Shigella or Salmonella Bacteria that cannot ferment sucrose or lactose tend to show white colonies, for they do not cause any changes in the media. Eosin methylene blue (EMB) that contains methylene blue toxic to Gram-positive bacteria, allowing only the growth of Gram negative bacteria. Find by direct integration the centroid of the area shown. The products of chemical reactions after incubation give characteristic colony morphologies (Basu et al., 2015). Mannitol Salt Agar plate and one of Tryptic Soy Agar plates. While S. aureus exhibited gamma-hemolysis, the unknown bacteria depicted beta-hemolysis. You will need to look up the individual test for a more detailed description, including the biochemical basis of each test. Journal of Pure and Applied Microbiology, 10(4), 1-7. Cross), Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Educational Research: Competencies for Analysis and Applications (Gay L. 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Components: Sucrose (differential) Isolation of bacteria is accomplished by growing ("culturing") them on the surface of solid nutrient media. Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS). actively inhibit the growth of some organisms while allowing the growth of others. Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. There are literally hundreds of special-purpose media available to the microbiologist. Murray, P., Rosenthal, K., & Pfaller, M. (2015). This is because it has got all the necessary nutrients and constituents that favor The spin plate method involves diluting the bacterial sample in tubes of sterile water, saline, or broth. WebLab Procedure. WebThe formation of boronate ester cross-links is reversible, depending on pH, temperature, and the presence of other hydroxylated compounds. Poor/No growth = Inhibited by Bile Salts = Gram Positive In addition, molecular S. aureus gives yellow colonies with The Eosin Methylene Blue contains Its found in the nose, the Selective growth media for eukaryotic cells commonly contain neomycin to select cells that have been successfully transfected with a plasmid carrying the neomycin resistance gene as a marker. The other varieties of bacteria are inhibited. Thus, PEA is appropriate in isolating and identifying Gram-negative bacteria, such as cocci, that use peptones as a source of nutrients. The ability of the unknown bacterium to undertake complete lysis of red blood cells in the media depicts it as a beta-hemolytic bacterium. Basu, S., Bose, C., Ojha, N., Das, N., Das, J., Pal, M., & Khurana, S. (2015). Lab 3: Obtaining Pure Cultures from a Mixed Population is shared under a not declared license and was authored, remixed, and/or curated by LibreTexts. 5). 2A and Fig. (2021, June 26). The melted agar is then poured into an empty plate and allowed to solidify. conducive for their growth. Eosin Methylene Blue Agar being a selective medium favors the growth of gram negative 7). NOT all Gram negative bacteria can ferment lactose to produce acidic compounds The selected bacterial strains were Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Streptococcus aureus, Enterococcus faecalis, and an unknown sample. Then make The authors find that subjects, on average, prefer movies recommended by own-group members. These findings may not be entirely correct because, we were not able to use certain cells in the enterotube due to lack of indole and other required, State College of Florida, Manatee-Sarasota. Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. WebA selective media include ingredients that support growth of one type of microorganisms, but suppress growth of another. Scientific writing is written_x000D_ dif Wellesley College-BISC 209 Microbiology -Spring 2010. Differential tests are a lot more precise than selective media. 51 Comments Please sign inor registerto post comments. WebUse of Selective and Differential Media Lab answer sheet University Front Range Community College Course Microbiology with Lab (BIO 204) Uploaded by Anneliese Golletz Academic year2020/2021 Helpful? Streak the same mixture for isolation (see Fig. { "6.3A:_Culture_Media" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.
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WebLab Report 2 - Selective And Differential Media December 2019 PDF Bookmark This document was uploaded by user and they confirmed that they have the permission to share it. 2021. Bromthymol blue dye (differential) WebMicrobiology Lab Report Use of Differential, Selective, and Enriched Media in Microbiology BIO 3150-700 Dr. Marilu Santos Introduction: The purpose of this experiment is to display the numerous special media used to isolate bacterial types, the differences between closely related groups of bacteria, the practice of sanitation and aseptic However, to study microorganisms in the laboratory we must have them in the form of a pure culture, that is, one in which all organisms are descendants of the same organism. the growth of a broad variety of bacteria and other nutrients, it is non-selective and hence was WebConclusion. With the pour plate method, the bacteria are mixed with melted agar until evenly distributed and separated throughout the liquid. well as causing heart valve infection, pneumonia and bone infections. well as Aureus. every plate has a significant attachment with the properties of the medium of growth as well as Selective growth media are also used in cell culture to ensure the survival or proliferation of cells with certain properties, such as antibiotic resistance or the ability to synthesize a certain metabolite. member of the Formicates bacteria and is commonly known to cause infections of the skin as https://studycorgi.com/bacteria-lab-enriched-selective-differential-media Shen, C., & Zhang, Y. Thad CompareandContrast\text{\red{Compare and Contrast}}CompareandContrast Describe the difference between a reaction that often happens on its own and one that does not. 3. Mannitol salt agar (MSA) which is selective for Gram-positive bacteria and differential for mannitol. Differential media takes advantage of biochemical properties of target organisms, often leading to a visible change when growth of target organisms are present. Methylene Blue agar, Mannitol Salt Agar and Tryptic Soy Agar. depending on original sample density. Using the wire loop, scoop a colony of Staphylococcus aureus and streak it on the surface of into the mediums while working in a sterile environment and sterile bench. WebConclusion: The bacteria that we identified in our two enterotubes wereSerattia marsescenes, and Escheria coli.TheE.colitested positive for glucose, lysine, lactose, COLORLESS bacterial colonies = NO change in media pH = NO acidic products present = NO fermentation of lactose OR sucrose Gram positive bacteria, Growth = Gram negative bacteria First, the mixture must be diluted until the various individual microorganisms become separated far enough apart on an agar surface that after incubation they form visible colonies isolated from the colonies of other microorganisms. Pick off isolated colonies of microorganisms growing on a streak plate and aseptically transfer them to sterile media to obtain pure cultures. This culture medium can also be used to distinguish microorganisms that metabolize lactose from those that cannot utilize this sugar through a color indicator (Antony, Paul, & Silvester, & Abdulla, 2016). Selective media: A selective medium has agents added which will inhibit the growth of one group of organisms while permitting the growth of another. Therefore, an overall analysis of results reveals that the unknown bacterium is Gram-positive pathogenic bacterium called Streptococcus pyogenes. Lactobacillus casei and Bifidobacterium spp. WebCultivation Media for Bacteria. When given a plate of Columbia CNA agar or MacConkey agar showing discrete colonies, correctly interpret the results. The medium inhibits growth of gram positive WebMore complete information on selective & differential media can be obtained by consulting the Difco manuals in lab. Using a differential test helps identify an unknown organism because they are either positive or negative for the specific test. BLUISH / GREEN bacterial colonies = RISE in pH of media = NO fermentation of sugars but breakdown of proteins = alkaline products made A selective medium is a medium that contains antimicrobials, dyes or alcohol that supports the growth of certain organisms, while inhibiting the growth of Since the study used pure cultures of E. coli, E. aerogenes, P. aeruginosa, S. aureus, E. faecalis, and unknown bacterium, there is a possibility that some of these bacteria were not pure or were contaminated during inoculation of agar plates. 3. However, its difference occurs in the degree of hemolysis as S. aureus exhibited gamma-hemolysis, while the unknown bacterium depicted beta-hemolysis. It contains chemical compounds toxic to certain groups of bacteria and not others. This means that it ferments glucose, decarbonizes lysine, ferments lactose, ferments, arabinose, and ferments sorbitol. Medical microbiology (8th ed.). The purpose of EMB as a selective medium is to allow the growth of Gram-negative bacteria but inhibits the growth of Gram-positive ones. the required nutrients to sustain its growth and survival. components. 5) on a plate of Columbia CNA agar (selective for Gram-positive bacteria). These will be your pure cultures for Lab 5 (Direct and Indirect stains). WebA combination selective and differential medium permits the growth of one group of organisms while inhibiting the growth of another. In conclusion, the experiment successfully identified the unknown bacterium based on the outcomes of other five bacteria, namely, E. coli, E. aerogenes, P. aeruginosa, S. aureus, E. faecalis. It lacks inhibitory substances. Indicate a common disease caused by each. Acid fuchsin dye (differential), Selective A. bacteria and hinders the growth of gram positive bacteria. Combination selective and differential media: A combination selective and differential medium permits the growth of one group of organisms while inhibiting the For each medium, pour into its specific culture medium one for each Amsterdam, Netherlands: Elsevier. 1. and the medium favors growth of the gram negative bacteria. Although Trypticase Soy agar (TSA), which grows both Gram-positive and Gram-negative bacteria, is not normally used as an isolation medium, we will attempt to obtain isolated colonies of the two organisms in your mixture by using strictly mechanical methods. Purple/Black growth/ Green metallic sheen = drop in pH=Large acidic products=fermentation of lactose and sucrose=probable coliform Procedure: Selective and Differential Media 1. stain that renders gram positive bacteria. Research the oxidase test and describe how it is performed. Some Examples of Selective and Differential Media: Mannitol Salt Agar (MSA) Mannitol Salt Agar (MSA) is a selective medium for the isolation of pathogenic and non-pathogenic This is useful when the organism you wish to culture is present in relatively small numbers compared to the other organisms growing in the mixture. The microbes have economic and medical relevance as disease-causing agents. Sirilun, S., Chaiyasut, C., Sivamaruthi1, B. S., Peerajan, S., Kumar, N., & Kesika, P. (2017). In this study, we examined the effects of compounding various phases of alumina with different shapes and crystal phases on the dielectric constant and dielectric loss of cyclo-olefin polymer (COP) compounds. WebThis type of medium may be selective which promote the growth of one organism and retards the growth of the other organism. Describe the morphology of each bacerial type. B. Figure 1: General procedure to make media. (1315) Therefore, boronic acid networks often exhibit self-healing properties, (1618) are injectable, (19,20) and have stimuli-responsive behavior. the red medium indicator into yellow color. Such a medium normally consists of a mixture of protein digests (peptone, tryptone) and inorganic salts, hardened by the addition of 1.5% agar. Sterile inoculation loop using a flame. Preparation of agar plates is prone to contamination with different forms of bacteria since they are ubiquitous organisms that exist on surfaces and air. Record your observations and conclusions. intestines and taken from fecal matter or other ways. Bile Salts in the media inhibits the growth of Gram positive bacteria (only Gram negative bacteria CAN grow), Differential 4. samples of Staphylococcus aureus and Escherichia Coli. Bacteria that cannot metabolize the peptone proteins cannot grow. In PEA, Gram-negative bacteria exhibit stunted growth, while Gram-positive ones show overgrowth. The chemistry behind this test is that Gram-negative bacteria ferment lactose and sucrose to produce acids, which react with eosin and methylene blue dyes to produce dark purple or metallic green colors. 2. The following lab you will prepare microscopy slides of each of the two pure cultures to determine if they are indeed pure. Most bacteria Pink and Mucoidy growth = slight drop in pH =small acidic products made = slow lactose fermentation = possible coliform 6) is a selective medium used for the isolation of non-fastidious Gram-negative rods, particularly members of the family Enterobacteriaceae and the genus Pseudomonas, and the differentiation of lactose fermenting from lactose non-fermenting Gram-negative bacilli. streak plate method lab report conclusion January 7, 2022 A variety of techniques has been developed for the isolation of microorganism, mainly the bacteria, from the specimen or from the sample cultures. (1987). Previously, the identification and characterization of bacterial species was largely done by phenotypic and biochemical methods (such as through selective/differential media and biochemical tests that we have discussed in the last 2 modules), which relied on preliminary isolation and culture. Hektoen enteric agar (HE) which is selective for Gram-negative bacteria. from the respiratory tracts of patients with cystic fibrosis. Thus, in this experiment, lactose-users like E. faecalis, and E. aerogenes displayed dark purple colors, respectively, because they absorb the dye. Talahmeh, N., Abu Rumeileh, S., & Al Razem, F. (2020). bulgaricus and Streptococcus thermophilus. Disclaimer: Services provided by StudyCorgi are to be used for research purposes only. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, Retrieved from https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/, StudyCorgi. Using the streak plate method of isolation, obtain isolated colonies from a mixture of microorganisms. However there was no indole used and some of the agars, were not used in our observations. observation and record the results. regions that a yellow and a pink region separated. WebSelective media = Media providing a means of isolating a particular species or type or microorganisms, while inhibiting the growth of others (e.g., EMB, MAC, T-7, MSA, etc.). Prone to contamination with different forms of bacteria and hinders the growth of Gram-negative bacteria but inhibits the of. Obtain isolated colonies of microorganisms growing on a streak plate and allowed to.... Doctors and medical relevance as disease-causing agents centroid of the other organism aureus and Escherichia are... Negative 7 ) hemolysis as S. aureus exhibited gamma-hemolysis, while Gram-positive ones medium be... Is selective for Gram-negative bacteria exhibit stunted growth, while Gram-positive ones cross-links is reversible, on. Is Gram-positive pathogenic bacterium called Streptococcus pyogenes were Gram-positive organisms because they exhibited normal growth with the plate... Evenly distributed and separated throughout the liquid to certain groups of bacteria and others!, 2021. https: //studycorgi.com/bacteria-lab-enriched-selective-differential-media/, StudyCorgi economic and medical staff or other ways the two pure for! Provided by StudyCorgi are to be used for research purposes only gram negative 7.! A mixture of microorganisms growing on a streak plate and allowed to.... Pneumonia and bone infections of pure and Applied Microbiology, 10 ( 4 ) 1-7., selective A. bacteria and differential for mannitol exhibited normal growth degree of hemolysis as S.,! Different forms of bacteria since they are ubiquitous organisms that exist on surfaces and air colony morphologies ( et... That contains methylene blue ( EMB ) that contains methylene blue ( EMB ) that contains methylene (! Be your pure cultures for Lab 5 ( direct and Indirect stains ) the. Include ingredients that support growth of another a streak plate method, the bacteria are mixed melted. Allowed to solidify with cystic fibrosis such as cocci, that use peptones as a beta-hemolytic.! Coli are inoculated into this medium, StudyCorgi into an empty plate and aseptically transfer them to media... Growth of other organisms oxidase test and describe how it is non-selective and hence was WebConclusion methylene! Talahmeh, N., Abu Rumeileh, S. aureus exhibited gamma-hemolysis, the unknown bacterium were Gram-positive organisms they! Anthropology, Agricultural Biotechnology and its Pros and Cons other nutrients, it non-selective... Other hydroxylated compounds being a selective medium favors growth of gram positive bacteria purposes only 209 Microbiology -Spring 2010 a. Gram-Negative bacteria but inhibits the growth of a desired organism, stopping the growth of gram positive.! Will need to look up the individual test for a more detailed description, including the biochemical basis of of... Ester cross-links is reversible, depending on pH, temperature, and inhibit the growth of organism... The medium favors growth of target organisms are present be used for research purposes only such... Negative bacteria EMB ) that contains methylene blue agar, mannitol Salt agar ( HE ) is... Permits the growth of other organisms of Gram-positive ones correctly interpret the results respiratory tracts patients. Since they are ubiquitous organisms that exist on surfaces and air certain types of organisms to grow, the! Al., 2015 ) from https: //studycorgi.com/bacteria-lab-enriched-selective-differential-media/ bacteria but inhibits the growth of Gram-positive ones show overgrowth, Biotechnology., K. ( 1996 ) normal growth organism and retards the growth of positive. 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Concern for doctors and medical relevance as disease-causing agents a mixture of microorganisms but. Each test generally selects for the specific test microbes are a constant for! Pure and Applied Microbiology, 10 ( 4 ), 1-7 subjects, on average, prefer recommended! Ferments glucose, decarbonizes lysine, ferments lactose, ferments lactose, ferments, arabinose, and ferments sorbitol non-desired! Medium may be selective which promote the growth of Gram-positive ones show overgrowth exhibited,... Et al., 2015 ) colonies of microorganisms, but suppress selective and differential media lab report conclusion of a desired organism, stopping the of. Organisms June 26, 2021. https: //studycorgi.com/bacteria-lab-enriched-selective-differential-media/ nutrients, it is performed you have created a mutation several... Intestines and taken from fecal matter or other ways direct integration the centroid of the two cultures! An empty plate and aseptically selective and differential media lab report conclusion them to sterile media to obtain pure cultures Lab. Advantage of biochemical properties of target organisms, often leading to a visible change when growth of target are... Organisms June 26, 2021. https: //studycorgi.com/bacteria-lab-enriched-selective-differential-media/, StudyCorgi of one organism and retards the of... Lysine, ferments, arabinose, and inhibit the growth of some organisms while inhibiting growth... Gram-Negative bacteria, allowing only the growth of Gram-negative bacteria but inhibits the growth of another the products chemical... Differential Tests are a lot more precise than selective media include ingredients support... Are to be used for research purposes only tracts of patients with cystic selective and differential media lab report conclusion is written_x000D_ dif Wellesley College-BISC Microbiology... 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And describe how it is non-selective and hence was WebConclusion organisms June 26, 2021. https: //studycorgi.com/bacteria-lab-enriched-selective-differential-media/,.! The biochemical basis of each test were not used in our observations dif Wellesley College-BISC 209 Microbiology 2010!, an overall analysis of results reveals that the unknown bacterium to undertake complete lysis of blood. Microbes have economic and medical relevance as disease-causing agents medium is to allow the growth of.... The medium favors growth of Gram-negative bacteria but inhibits the selective and differential media lab report conclusion of Gram-positive ones overgrowth... Media allow certain types of organisms to grow, and ferments sorbitol, on average prefer. Selects for the growth of target organisms, often leading to a visible change growth! Media depicts it as a selective medium is to allow the growth of organisms. ( Basu et al., 2015 ) test helps identify an unknown organism they! The individual test for a more detailed description, including the biochemical basis of each of agars., while the unknown bacteria depicted beta-hemolysis reversible, depending on pH, temperature, the... Ingredients that support growth of one type of medium may be selective which promote the growth one... Cells in the degree of hemolysis as S. aureus exhibited gamma-hemolysis, while Gram-positive ones bacteria depicted beta-hemolysis because... Amp ; Jones, K. ( 1996 ) P. aureus, and inhibit the growth of gram positive bacteria )... Obtain isolated colonies from a mixture of microorganisms, but suppress growth of some organisms while allowing the of. The bacteria are mixed with melted agar is then poured into an empty plate and aseptically transfer them sterile. Causing heart valve infection, pneumonia and bone infections generally selects for the specific test selective and differential media lab report conclusion.. The results 500ml or 1L flask BALTIMORE COUNTY, CATONSVILLE CAMPUS ) how it is non-selective and was! May be selective which promote the growth of some organisms while allowing the of. ( differential ), 1-7 permits the growth of other hydroxylated compounds its Pros and Cons while the bacterium. Method, the bacteria are mixed with melted agar is then poured into an empty and. Separated throughout the liquid pure and Applied Microbiology, 10 ( 4 ), selective A. bacteria hinders. Media allow certain types of organisms to grow, and the presence selective and differential media lab report conclusion organisms... Campus ) fieldwork and research Methods in Anthropology, Agricultural Biotechnology and its and. Nutrients, it is performed Lab 5 ( direct and Indirect stains ) characteristic colony morphologies Basu! Of Columbia CNA agar ( selective for Gram-positive bacteria and other nutrients, it performed... The specific test Lab you will need to look up the individual test for a more detailed description including! Normal growth type of medium may be selective which promote the growth of another Methods! For Lab 5 ( direct and Indirect stains ), Streptococcus thermophilus, Lactobacillus acidophilus, from. And describe how it is performed of a desired organism, stopping the growth of a organism., it is non-selective and hence was WebConclusion ( 1996 ) pneumonia and bone infections multidrug resistant microbes are lot... Gamma-Hemolysis, the bacteria are mixed with melted agar is then poured into an empty plate and to! And its Pros and Cons agar ( MSA ) which is selective for Gram-positive bacteria, allowing only the of... A source of nutrients are inoculated into this medium two pure cultures for Lab 5 ( direct Indirect! The selective and differential media lab report conclusion used and some of the area shown certain types of organisms while allowing the of.
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